LIC Generator, a resource to generate primers for Ligation Independent Cloning

Restriction no more !

Links
Primer design for:
  • LIC-PCR
  • Mixed-PCR
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  • Detection of internal LIC sequences
  • Primer design at Clontech (In-fusion)
  • All about LIC
  • LIC protocols
  • sLIC at Bitesize Bio

Introduce tags or protease cleavage sites into your insert and vector

1- Choose the features of your LIC1 sequence (5' to your insert)

Tag/Protease

2- Choose the features of your LIC2 sequence (3' to your insert)

Tag/Protease
Contact: marie(-)claire(dot)dagher(at)ujf(dash)grenoble(dot)fr
Marie-Claire Dagher - Laboratoire TIMC-IMAG, équipe TheREx, UMR CNRS 5525, 38043 Grenoble Cedex 9 France   logo cnrs    logo Universite Joseph Fourier  Acknowledgements:  logo cea